Western Blot:Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis.
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137
Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137 Report ll OPEN ACCESS
Recombinant:Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis.
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137
Article Title: CDK4 Phosphorylates AMPKα2 to Inhibit Its Activity and Repress Fatty Acid Oxidation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-ACC Cell Signaling Technology Cat#3662 Rabbit polyclonal anti-Phospho-ACC Cell Signaling Technology Cat#3661 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat2532 Rabbit monoclonal anti-Phospho-AMPKa(Thr172)(40H9) Cell Signaling Technology Cat#2535 Mouse monoclonal anti-Myc-Tag (9B11) Cell Signaling Technology Cat#2276 Rabbit monoclonal anti-Phospho Rb (ser780)(D59B7) Cell Signaling Technology Cat#8180 Rabbit polyclonal anti-Cdk4 (C-22) Santa Cruz biotechnology Sc-260 Mouse monoclonal anti-Rb (C-2) Santa Cruz biotechnology Sc-74562 Goat polyclonal anti-AMPK a2 (C-20) Santa Cruz biotechnology Sc-19131 Goat polyclonal anti-Actin (C11) Santa Cruz biotechnology Sc-1615 Rabbit polyclonal anti-Myc-Tag Abcam Ab9106 Mouse monoclonal anti-a-Tubulin Sigma-Aldrich T6199 Chemicals, Peptides, and Recombinant Proteins LY2835219 MedChemExpress HY-16297 PD0332991 MedChemExpress HY-50767 LEE011 MedChemExpress HY-15777 A769662 MedChemExpress HY-50662 Compound C MedChemExpress HY-13418 FCCP Sigma Aldrich C2920 Antimycine A Sigma Aldrich A8674 CDK4/CycD3 ProQinase 0142-0373-1 CDK4/CycD1 ProQinase 0142-0143-1 Recombinant kinase dead AMPK trimers (a2b2g1) DG. ..
Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137 Report ll OPEN ACCESS
Thin Layer Chromatography:Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis.
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137
Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137 Report ll OPEN ACCESS
SYBR Green Assay:Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis.
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137
Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137 Report ll OPEN ACCESS
Polymerase Chain Reaction:Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis.
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137
Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137 Report ll OPEN ACCESS
cDNA Synthesis:Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis.
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137
Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137 Report ll OPEN ACCESS
Lysis:Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis.
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137
Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137 Report ll OPEN ACCESS
Solvent:Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis.
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137
Article Title: Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis
Article Snippet: T.C.W. has an immediate family member working for Pfizer, Inc. T.C.W. and R.V.F. have a sponsored research agreement with Pfizer, Inc. 8 Cell Reports 33, 108348, November 3, 2020 Received: April 30, 2020 Revised: August 29, 2020 Accepted: October 13, 2020 Published: November 3, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Glut1 Cell Signaling Technology Cat#12939 Mouse monoclonal anti-Glut4 Cell Signaling Technology Cat# 2213 Rabbit polyclonal anti- Na, K-ATPase Cell Signaling Technology Cat# 3010 Rabbit polyclonal anti-tyrosine hydroxylase Abcam Cat# ab112 Rabbit monoclonal anti-Akt Cell Signaling Technology Cat# 4691 Rabbit monoclonal anti-phospho-Akt (Ser473) Cell Signaling Technology Cat# 4060 Rabbit polyclonal anti-AMPKa Cell Signaling Technology Cat# 2532 Rabbit monoclonal anti-phospho-AMPKa (Thr172) Cell Signaling Technology Cat# 2535 Rabbit polyclonal anti-UCP1 Abcam Cat# ab10983 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 Rabbit polyclonal anti-PGDS Abcam Cat# ab182784 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174S Mouse monoclonal anti-a-Tubulin Sigma-Aldrich Cat# T9026 Chemicals, Peptides and Recombinant Proteins 1,2-Dioleoyl-rac-glycerol Sigma-Aldrich Cat# D8394 Oleoyl coenzyme A lithium salt Sigma-Aldrich Cat# O1012 Oleoyl [14C] Coenzyme A American Radiolabeled Chemicals, Inc. Cat# ARC 0527 Oleic acid [14C] American Radiolabeled Chemicals, Inc. Cat# ARC 0297 Thin Layer Chromatography Plates Analtech Cat# P43911 Insulin Lilly Corporation Humulin R (U 100) CL 316,243 hydrate Sigma-Aldrich Cat# C5976 Glycogen from bovine liver Sigma-Aldrich Cat# G0885 Protease inhibitors Roche Cat# 11873580001 Phospho(enol)pyruvic acid monopotassium salt Sigma-Aldrich Cat# P7127 Pyruvate Kinase from rabbit muscle Sigma-Aldrich Cat# P1506 b-Nicotinamide adenine dinucleotide Sigma-Aldrich Cat# N8129 Critical Commercial Assays Power SYBR Green PCR Master Mix Life Technologies Cat # 4368706 iScript cDNA Synthesis Kit Biorad Cat # 170-8891 RNeasy Mini Kit QIAGEN Cat # 74106 QIAzol Lysis Reagent QIAGEN Cat # 79306 QIAshredder QIAGEN Cat# 79656 RNase-Free DNase Set QIAGEN Cat# 79254 SuperSignal West Pico ThermoFisher Scientific Cat# 34580 SuperSignal West Femto ThermoFisher Scientific Cat# 34095 Glycogen Assay Kit II Abcam Cat# ab169558 Infinity Triglycerides Reagent Thermo Fisher Cat # TR22421 Infinity Cholesterol Reagent Thermo Fisher Cat # TR13421 Free Glycerol Reagent Sigma-Aldrich Cat # F6428 HR Series NEFA-HR(2) Color Reagent A FUJIFILM Medical Systems Cat # 999-34691 HR Series NEFA-HR(2) Solvent A FUJIFILM Medical Systems Cat # 995-34791 (Continued on next page) Cell Reports 33, 108348, November 3, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HR Series NEFA-HR(2) Color Reagent B FUJIFILM Medical Systems Cat # 991-34891 HR Series NEFA-HR(2) Solvent B FUJIFILM Medical Systems Cat # 993-35191 Experimental Models: Organisms/ Strains Dgat1flox/flox mice (Shih et al., 2009); The Jackson Laboratory Cat # 017322 Dgat2flox/flox mice (Chitraju et al., 2019); The Jackson Laboratory Cat # 033518 Ucp1-Cre mice (Kong et al., 2014); The Jackson Laboratory Cat # 024670 BA-DGAT KO mice This paper N/A Other Chow-diet PicoLab Rodent Diet 20 Cat # 5053 Breeder’s-diet PicoLab Rodent Diet 20 Cat # 5058 High-fat-diet Envigo Cat # TD.88137 Report ll OPEN ACCESS
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